A simple method for isolating and culturing single cancer stem cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 802-806, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-268994
ABSTRACT
<p><b>OBJECTIVE</b>To develop an effective method for isolating and culturing single cancer stem cells.</p><p><b>METHODS</b>The capillary glass tube was stretched on fire and connected to a sterile plastic tube to prepare the single cell separation apparatus. Single SW480 cell clone spheres in serum-free culture were marked with CD133 and CK7, and the single cancer stem cells were separated and cultivated in 96-well plates or microdrop covered by paraffin.</p><p><b>RESULTS</b>SW480 cell clone formation rate was about 1.04%, and the cell clone spheres highly expressed CD133 with low CK7 expression. The isolation of the single cancer stem cells showed a success rate of 98.99% using the separation device. The cell division profile was comparable between the cell cultures in microdrop and 96-well plates in the initial 2 cell divisions (P>0.05), whereas prolonged cell division occurred afterwards in the microdrop culture as compared to 96-well plate culture. The cell population expansion of the single cancer stem cells was similar between microdrop culture (11.5%, 22/192) and 96-well plate culture (9.2%, 17/184) (P>0.05).</p><p><b>CONCLUSIONS</b>Single SW480 cells can develop into cancer stem cell spheres. Microdrop culture is convenient and stable, and can be the primary choice for single cancer stem cell culture.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Neoplastic Stem Cells
/
Cell Separation
/
Cell Culture Techniques
/
Cell Biology
/
Cell Line, Tumor
/
Methods
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article
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