Antitumor effect of a triple negative breast cancer-dendritic cell fusion vaccine / 南方医科大学学报
Journal of Southern Medical University
;
(12): 778-783, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-268999
ABSTRACT
<p><b>OBJECTIVE</b>To test the antitumor effect of a human triple-negative breast cancer cell-dendritic cell (DC) fusion vaccine.</p><p><b>METHODS</b>DCs were isolated from fresh peripheral blood of healthy donors. The fusion vaccine was prepared by fusing the DCs and MDA-MB-231 cells via electrofusion. The morphology of the vaccine was identified under inverted fluorescence microscope and the phenotypes were analyzed with flow cytometry. The production of interleukin-12 (IL-12) and interferon-γ (IFN-γ) by the fusion cells was assessed using ELISA. A CCK-8 kit was used to examine the effect of the vaccine in stimulating the proliferation and cytotoxicity of autologous T lymphocytes.</p><p><b>RESULTS</b>The DCs isolated from peripheral blood mononuclear cells highly expressed CD83, CD86, CD11c and HLA-DR on the cell surface. The fusion cells were irregular in shape and coexpressed the phenotypes of DCs and MDA-MB-231 cells. The fusion cells possessed a strong ability to stimulate the proliferation of T lymphocytes in vitro. Compared with the control group, the fusion vaccine showed a stronger antitumor effect against the breast cancer cells.</p><p><b>CONCLUSION</b>The triple-negative breast cancer-DC fusion vaccine prepared by electrofusion can stimulate the proliferation of T lymphocytes and induces strong cytotoxicity of the T cells against breast cancer cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Dendritic Cells
/
Breast Neoplasms
/
Lymphocyte Activation
/
T-Lymphocytes, Cytotoxic
/
Cell Fusion
/
Interferon-gamma
/
Interleukin-12
/
Cancer Vaccines
/
Cell Line, Tumor
/
Allergy and Immunology
Type of study:
Prognostic study
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article
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