Preparation of a monoclonal antibody against fusion protein preM/EIII of WNV / 南方医科大学学报
Journal of Southern Medical University
;
(12): 742-745, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-269007
ABSTRACT
<p><b>OBJECTIVE</b>To prepare a monoclonal antibody (mAb) against the fusion protein preM/EIII of West Nile virus (WNV) for clinical detection of WNV.</p><p><b>METHODS</b>Sp2/0 cells were fused with the spleen cells of BALB/c mice immunized with the recombinant fusion protein preM/EIII expressed in E. coil to obtain the hybridoma cell line that secreted preM/EIII mAb. The hybridoma cells were injected into the peritoneal cavity of BALB/c mice and the ascites was collected and purified. The specificity and titer of the obtained mAb were determined using ELISA and Western blotting.</p><p><b>RESULTS</b>One hybridoma cell line secreting preM/EIII mAb, named ME1, was obtained. The titer of the purified mAb was 10(-6). Identified as a mAb of the Ig subclass IgG1, ME1 was capable of specific reactions with preM/EIII protein and WNV without cross-reactions with other viruses such as JEV, SLEV, YFV and DENV. The accuracy of clinical testing of MNV with ME1 was 97.78%.</p><p><b>CONCLUSION</b>The mAb against preM/EIII obtained have a high specificity and accuracy in clinical testing of MNV and can be used in clinical diagnosis of MNV infection.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
West Nile virus
/
Viral Fusion Proteins
/
Blotting, Western
/
Cross Reactions
/
Allergy and Immunology
/
Hybridomas
/
Mice, Inbred BALB C
/
Antibodies, Monoclonal
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article
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