Expression of leukocyte-associated Ig-like receptor-1 in children with immune thrombocytopenia / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 370-374, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-269470
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of leukocyte-associated Ig-like receptor-1(LAIR-1) in children with immune thrombocytopenia (ITP), in order to explore the possible role of LAIR-1 in the pathogenesis of childhood ITP.</p><p><b>METHODS</b>Expression levels of LAIR-1 on CD4(+) T cells, CD8(+) T cells and CD19(+)CD20(+) B cells of peripheral blood were measured in 40 children with ITP by flow cytometry. Serum level of solubility LAIR-1 (sLAIR-1) was measured using ELISA. Real-time PCR was used to measure LAIR-1 mRNA expression. Thirty-two healthy children served as the control group.</p><p><b>RESULTS</b>The percentages of CD19(+)CD20(+) B cells in the ITP group were significantly higher than in the control group (P<0.05). In contrast, the percentage of CD4(+) T cells in the ITP group was significantly lower than in the control group (P<0.05). The expression levels of LAIR-1 on CD4(+) T cells and CD8(+) T cells were significantly lower in the ITP group than in the control group (P<0.05). Serum sLAIR-1 level and LAIR-1 mRNA expression in the ITP group significantly increased compared with the control group (P<0.05).</p><p><b>CONCLUSIONS</b>LAIR-1 expression on CD4(+) and CD8(+) T cells decreases and serum sLAIR-1 level increases in children with ITP, suggesting that LAIR-1 may play an important role in immune imbalance in these children.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Blood
/
RNA, Messenger
/
Receptors, Immunologic
/
CD4-Positive T-Lymphocytes
/
Purpura, Thrombocytopenic, Idiopathic
/
CD8-Positive T-Lymphocytes
/
Allergy and Immunology
/
Genetics
Limits:
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2014
Type:
Article
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