Your browser doesn't support javascript.
loading
Establishment of a cell culture model of corpus cavernosum smooth muscle cells derived from diabetic rats with erectile dysfunction / 南方医科大学学报
Journal of Southern Medical University ; (12): 351-354, 2010.
Article in Chinese | WPRIM | ID: wpr-269553
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the method for culturing corpus cavernosum smooth muscle cells (CCSMs) derived from diabetic rats with erectile dysfunction (ED) for the study of ED caused by diabetes.</p><p><b>METHODS</b>CCSMs were isolated from the corpus cavernosum of diabetic rats with ED and cultured using a modified method of adherent tissue culture. The cultured cells were identified by immunohistochemistry and the cell morphology and proliferation were observed.</p><p><b>RESULTS</b>The primary culture of CCSM was performed successfully, and the cells were seen to migrate from the small tissue pieces 3 days later, reaching nearly confluence in 16-18 days. A typical "hill-valley" growth pattern was noted in the cell passaging. Immunohistochemical staining for alpha-smooth muscle actin (alpha-SM-actin) and desmin yielded positive results in the cells.</p><p><b>CONCLUSION</b>The modified method for adherent tissue culture is convenient and reliable in establishing the in vitro cell culture model of CCSMs from diabetic rats with ED, and the cultured CCSMs display a faster proliferation than normal CCSMs. No obvious differences in the cell morphology can be found between diabetic and normal CCSMs under light microscope.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Penis / Physiology / Penile Erection / Cells, Cultured / Rats, Sprague-Dawley / Cell Culture Techniques / Cell Biology / Myocytes, Smooth Muscle / Diabetes Mellitus, Experimental Limits: Animals Language: Chinese Journal: Journal of Southern Medical University Year: 2010 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Penis / Physiology / Penile Erection / Cells, Cultured / Rats, Sprague-Dawley / Cell Culture Techniques / Cell Biology / Myocytes, Smooth Muscle / Diabetes Mellitus, Experimental Limits: Animals Language: Chinese Journal: Journal of Southern Medical University Year: 2010 Type: Article