Construction and biological activity study of human osteoprotegerin expressing adenoviral system / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 35-40, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-270042
ABSTRACT
Using the isolated total RNA from osteosacoma cell line MG63, the cDNA encoding human OPG was amplified by RT-PCR. A recombinant adenoviral vector carrying cDNA of OPG was constructed and OPG expression in mouse myoblast C2C12 cells was confirmed by Western blot and ELISA. The secreted expression of OPG protein persisted more than 6 weeks in vitro, and the growth of C2C12 cells infected by recombinant adenoviral were in good state. Osteoclasts derived from mouse bone marrow cells infected with recombinant adenoviral made less number of TRAP positive cells and resorption pits formed on dentine slices.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoclasts
/
Enzyme-Linked Immunosorbent Assay
/
Cell Line
/
Adenoviridae
/
Blotting, Western
/
Reverse Transcriptase Polymerase Chain Reaction
/
Osteoprotegerin
/
Genetic Vectors
/
Genetics
/
Metabolism
Limits:
Animals
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2003
Type:
Article
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