Isolation and identification of reprogramming genes related to the development of the rabbit reconstructed embryos / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 30-34, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-270043
ABSTRACT
By the method of single preimplantation embryos differential display polymerase chain reaction (SPEDDRT-PCR), 25 reprogramming cDNA fragments were obtained from single 2-cell, 8-cell embryos and blastula. After cloning and sequencing, five of them were identified by reverse-Northern and characterized with stage-specific expression during reconstructed embryo development. This results will help to isolate full length reprogramming genes and study their function during embryonic development.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Blastocyst
/
Blotting, Northern
/
Polymerase Chain Reaction
/
Gene Expression Regulation, Developmental
/
Embryonic Development
/
Embryo, Mammalian
/
Genetics
/
Metabolism
Type of study:
Diagnostic study
Limits:
Animals
/
Pregnancy
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2003
Type:
Article
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