Study on the culture of crown gall from Panax quinquefolium and the prouction of its secondary metabolites--Ginsenosides Re and Rg1 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 372-375, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-270050
ABSTRACT
It was clearly demonstrated that T-DNA of Agrobacterium tumefeciens Ti plasmid was integrated into the cells of crown gall in our experiment. This paper reported the influences of some kinds of physical-chemistry factors on the growth of crown gall of Panax quinquefolium and the production of its main active compounds--ginsenoside Re and ginsensoside Rg1. The results showed that White medium was the best one for ginsensoside Rg1 accumulation (0.095%) among the six media, but ginsensoside Re accumulation (0.194%) was the highest on the MS medium; The highest contents of ginsensoside Re (0.147%) and ginsensoside Rg1 (0.061%) were on the culture 36d and 32d after innoculum respectively; The optimum pH was 5.6 for ginsensoside Rg1 synthesis(0.054%), and 5.8 for ginsensoside Re synthesis(0. 184% ); The contents of ginsensoside Re and ginsensoside Rg1 was the highest in the inoculum of 4 g and 2 g/flask (FW) respectively. The result also indicated that the concentration of inositol in 0.05 g/L could obviously promote ginsensoside Re synthesis (0.182%), and in 0.30 g/L for ginsensoside Rg1 (0.055%).
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Plant Tumors
/
Agrobacterium tumefaciens
/
Cell Culture Techniques
/
Ginsenosides
/
Genetics
/
Panax
/
Hydrogen-Ion Concentration
/
Metabolism
/
Microbiology
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS