Cloning and sequence analysis of MYB transcriptional regulator SmP gene of Saussurea medusa Maxim / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 368-371, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-270051
ABSTRACT
A full-length cDNA encoding a MYB-related regulatory gene was isolated from a cDNA library prepared from mRNAs of the red line callus of S. medusa by TD-PCR. The cDNA, designated SmP, is 969 nucleotides long and has an open reading frame of 771 bp with a deduced amino acid sequence of 256 residues. The putative protein of SmP has two typical conversed R2R3-Myb DNA-binding domains in N-terminal and displays a rather high degree of similarity to OsMYB from rice and LBMI from tobacco, showing 73% and 70% identity within the DNA-binding domains. However, the C-terminal domain of the SmP protein does not show obvious similarity to any other known protein sequence. It is rich in hydrophilic amino acids, especially in serine residues (18.38%), partly organized in homopolymeric stretches, a feature often found in activation domain of transcription factors.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Plant Proteins
/
Transcription Factors
/
Molecular Sequence Data
/
Chemistry
/
Polymerase Chain Reaction
/
Amino Acid Sequence
/
Classification
/
Cloning, Molecular
/
Sequence Homology, Amino Acid
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2003
Type:
Article
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