The expression of growth hormone release factor gene carried with PLGA microspheres in muscle tissue and its effect on mouse growth / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 615-618, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-270076
ABSTRACT
Injectable sustained-release pcDNA3-GRF (1-32) microspheres were prepared by double emulsion-in liquid evaporation process,using biodegrable poly lactic-co-glycolic acid as carrier. The enrapment efficiency, mean particle size, drug content thus prepared were 69%, 2.20 microm, 8% and 70% respectively. The result of transfection in vivo showed that after 30 days, accumulative increased body weights on the group injected with pcDNA3-GRF (1-32) microspheres was significantly higher than those group injected with naked plasmid (12.87%), plasmid-empty microspheres (19.72%) and saline (58.58%) respectively. PCR and RT-PCR showed that the expression level of GRF gene on the group injected with pcDNA3-GRF (1-32) microspheres was the highest. GRF gene released by microspheres was still detected after 30 days. In conclusion, pcDNA3-GRF (1-32) microspheres have a controlled release effect and GRF gene could be successfully transfected into muscle cells of mouse by microspheres with higher efficacy and stronger biological function.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Polyglycolic Acid
/
Body Weight
/
DNA
/
Polymerase Chain Reaction
/
Growth Hormone-Releasing Hormone
/
Muscle, Skeletal
/
Lactic Acid
/
Genetics
/
Growth
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2004
Type:
Article
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