The labeling of 3T3-L1 preadipocyte cells with enhanced green fluorescent protein / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 607-609, 2004.
Article
in English
| WPRIM
| ID: wpr-270078
ABSTRACT
A cell model is desired for adipocyte differentiation investigation and for high-throughput screening of anti-obesity and anti-diabetes molecules from chemical resources due to the world wide epidemic of obesity and diabetes. In order to establish such a cell model, a plasmid of pPPARgamma2-promoter-EGFP was constructed by inserting a 660bp sequence of mouse PPARgamma2 promoter into the Ase I and Kpn I sites of pEGFP-N3 and transferred into 3T3-L1 preadipocyte cells. The cells were induced to differentiate and the expression of PPARgamma2 was detected by the microscopic observation of EGFP and by RT-PCR assays. The results showed that the EGFP gene expression patterns were similar to that of pPPARgamma2's, which indicated that the EGFP gene was transferred into the mouse 3T3-L1 preadipocyte cells, and its expression was under the control of pPPARgamma2 promoter. RT-PCR assays showed that the EGFP expression authentically represented the stable expression of PPARgamma2. In conclusion, a preadipocyte cell line expressing EGFP under the control of the promoter of adipocyte-specific expression gene PPARgamma2 was generated. The cell line provides a powerful approach for the research of adipocyte differentiation and for the high-throughput screening of anti-obesity and anti-diabetes chemicals.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stem Cells
/
Cell Differentiation
/
Promoter Regions, Genetic
/
Adipocytes
/
Cell Biology
/
3T3-L1 Cells
/
Green Fluorescent Proteins
/
PPAR gamma
/
Genetics
Limits:
Animals
Language:
English
Journal:
Chinese Journal of Biotechnology
Year:
2004
Type:
Article
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