Sequencing and bioinformatic analysis of NRDRiso cDNA / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 520-525, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-270094
ABSTRACT
This study describes the cDNA sequencing and the bioinformatic analysis of a novel NADP(H)-dependent retinol dehydrogenase/reductases isoform (NRDRiso). Based upon the concensus sequences of human and mouse NRDR coding region, we have identified a short 377 bp RT-PCR product from human liver tissue. The cDNA sequence of a NRDR isoform was then isolated using RACE approach and its sequence was analysed. The full-length cDNA is 1,003bp in length and was submitted to GenBank as NADP-dependent retinol dehydrogenase/reductase short isoform (NRDRiso). The open reading frames of NRDRiso cDNA is 525 bp.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Molecular Sequence Data
/
Base Sequence
/
Chemistry
/
Open Reading Frames
/
Chromosome Mapping
/
Amino Acid Sequence
/
Sequence Analysis, DNA
/
DNA, Complementary
/
Computational Biology
/
Alcohol Oxidoreductases
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2004
Type:
Article
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