Effects of nutrient conditions and fed-batch culture on CoQ10 production by Rhizobium radiobacter WSH2601 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 212-216, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-270111
ABSTRACT
The effects of the variables, including the concentrations of glucose, sucrose, corn steep liquor (CSL) and peptone, and the conditions of fed-batch culture, on CoQ10 fermentation by Rhizobium radiobacter WSH2601 were assessed. The results showed that the optimum concentrations of glucose, sucrose, CSL and peptone were 30 g/L, 40 g/L, 11 g/L and 16 g/L respectively. Addition of CSL and tomato juice stimulated the cell growth. CSL, L-methionine and isopentyl alcohol efficiently increased the biosynthesis of CoQ10. In a 7L fermentor, the fed-batch culture improved both cell growth and CoQ10 production compared to a batch culture; and suitable mixed feeding of CSL and sucrose enhanced CoQ10 yield to 52.4 mg/L. The DCW reached 26.4 g/L, an increase of 53% in comparison to that without feeding, and an increase of 24% to that feeding with sucrose only. The C/B value reached 2.38 mg/g(DCW), representing an increase of 33% to that of without feeding, and an increase of 26% to that of feeding with sucrose only.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Sucrose
/
Industrial Microbiology
/
Ubiquinone
/
Agrobacterium tumefaciens
/
Culture Media
/
Fermentation
/
Glucose
/
Metabolism
/
Methods
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2003
Type:
Article
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