Biosynthesis of poly (3-mercaptopropionate) and poly (3-mercaptopropionate-co-3-hydroxybutyrate) with recombinant Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 195-199, 2003.
Article
in English
| WPRIM
| ID: wpr-270114
ABSTRACT
Polythioesters newly emerged as a type of novel polymer and they have showed great potential for application in industries. In this study, genes of butyrate kinase (buk) and phosphotransbutyrylase (ptb) from Clostridium acetobutylicum, and poly (3-hydroxybutyrate) (PHB) synthase gene from Thiocapsa pfennigii were used for construction of a metabolic pathway to synthesize the polythioesters. When 3-mercaptopropionate and 3-hydroxybutyrate were fed, poly (3-mercaptopropoinate) [poly (3MP)] and poly(3-mercaptopropionate-co-3-hydroxybutyrate) [poly(3MP-co-3HB)] were synthesized by recombinant Escherichia coli JM109 (pBPP1) harboring the constructed metabolic pathway. Results indicated clearly that all these genes are necessary for the synthesis of poly(3MP) and poly(3MP-co-3HB).
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphate Acetyltransferase
/
Plasmids
/
Polymers
/
Spectrophotometry, Infrared
/
Acyltransferases
/
Chemistry
/
Phosphotransferases (Carboxyl Group Acceptor)
/
3-Hydroxybutyric Acid
/
Escherichia coli
/
Genetics
Language:
English
Journal:
Chinese Journal of Biotechnology
Year:
2003
Type:
Article
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