Effects of paroxetine on protein kinase PKA, PKC and CaMKII activity in different brain regions in a rat depression model / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1223-1225, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-270169
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effects of paroxetine on protein kinase PKA, PKC and CaMKII activities in different brain regions in a rat model of depression.</p><p><b>METHODS</b>Thirty-six adult male SD rats were randomized into 6 groups, including one control group (I) and 5 groups of depression model established by forcing the rats to swim for 4 weeks. The 5 depression groups received no treatment (II) or were treated with paroxetine at a single dose (III), for a week (IV), 2 weeks (V) or 4 weeks (VI). The radioactivity of PKA, PKC and CaMKII in the hippocampus and prefrontal cortex was quantitatively measured using a liquid scintillation counter.</p><p><b>RESULTS</b>In the rat hippocampus, PKA and CaMKII activities were significantly lower in groups II, III, IV, and V than in groups I and VI (P<0.01 or P<0.05), but comparable between groups VI and I (P>0.05). PKC activity was significantly lower in group II than in group I (P<0.01), but showed no significant difference between the paroxetine-treated groups and group I (P>0.05). In the prefrontal cortex, the activity of PKA in groups I, II, III, and IV was similar (P>0.05), but all significantly lower than that in groups V and VI (P<0.01). PKC activity was significantly higher in groups II and III than that in group I and other paroxetine-treated groups (P<0.01), and similar between groups IV and I (P>0.05); groups V and VI had significantly lower PKC activity than group I (P<0.01). Group I had the highest CaMKII activity among the groups (P<0.01).</p><p><b>CONCLUSION</b>Chronic administration of paroxetine can reverse chronic stress-induced inhibition of PKA, PKC and CaMKII activity in rat hippocampus, while the effects of paroxetine on the protein kinases can be more complex in prefrontal cortex.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Brain
/
Protein Kinase C
/
Random Allocation
/
Paroxetine
/
Cyclic AMP-Dependent Protein Kinases
/
Depression
/
Disease Models, Animal
/
Calcium-Calmodulin-Dependent Protein Kinase Type 2
/
Hippocampus
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2008
Type:
Article
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