High-performance liquid chromatography-mass spectrometry for determining olmesartan in human plasma / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1104-1105, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-270200
ABSTRACT
<p><b>OBJECTIVE</b>To establish a simple and rapid HPLC-MS method for determining the contents of olmesartan in human plasma.</p><p><b>METHODS</b>Plasma were precipitated with trifluoroacetic acid, then analyzed on an HyPurity C(18) column (150 mm 2.1 mm, 5 microm). Samples at 40 degrees celsius;. The mobile phase consisted of water-methanol- acetonitrile(146026) with a flow rate of 0.22 ml/min.</p><p><b>RESULTS</b>The lower limit of qualification was 25 microg/L. The calibration curve was linear over the range of 25-3200 microg/L (r=0.9998), with the intra-day and inter-day RSD less than 15%.</p><p><b>CONCLUSION</b>The method is sensitive, rapid and suitable for the study of pharmacokinetics and bioavailability of olmesartan.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Mass Spectrometry
/
Tetrazoles
/
Blood
/
Calibration
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Angiotensin II Type 1 Receptor Blockers
/
Imidazoles
/
Methods
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2008
Type:
Article
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