Preparation and characterization of specific monoclonal antibodies against hexon of HAdV 3 / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To obtain the monoclonal antibody against hexon protein of human adenovirus.</p><p><b>METHODS</b>BALB/c mice were immunized with purified recombinant hexon protein, and the spleen cells of the mice were isolated and fused with myloma cells. Four hybridoma cell strains were screened by indirect ELISA and cultured, and the sensitivity, specificity and virus neutralizing activity were analyzed with ELISA, Western blotting and neutralizing test.</p><p><b>RESULTS</b>The mouse ascites produced by these hybridoma cells contained specific monoclonal antibodies against hexon protein of human adenovirus as identified by ELISA and Western blot, and the antibody generated by 4C6 strain showed human adenovirus type 3-neutralizing activity.</p><p><b>CONCLUSION</b>The monoclonal antibodies against hexon protein with high specificity have been successfully obtained, and these antibodies can be useful in developing assays for early diagnosis of HAdV3 infection and also in study of therapeutic drugs of the infection.</p>