Screening peptides binding specifically to large intestinal cancer LoVo cells from phage random peptide library / 南方医科大学学报
Journal of Southern Medical University
;
(12): 986-990, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-270229
ABSTRACT
<p><b>OBJECTIVE</b>To screen the polypeptides specifically binding to human large intestinal cancer LoVo cells from a phage-displayed peptide library for potential use as targeting vectors for large intestinal cancer therapy.</p><p><b>METHODS</b>With the LoVo cells as the target cells and human normal large intestinal mucosal epithelial cells as the absorber cells for subtraction biopanning from a c7c phage-display peptide library, the positive phage clones were identified by enzyme-linked immunosorbent assay (ELISA) and immunofluorescence detection. The amino acid sequences of the identified peptides were deduced by DNA sequencing.</p><p><b>RESULTS</b>After 3 rounds of screening, 5 positive phage clones showing specific binding to LoVo cells and containing conserved motif RPMP were obtained from the 20 randomly selected clones.</p><p><b>CONCLUSION</b>Specific peptide against large intestinal cancer cells can be obtained from a phage-display peptide library for use as potential vectors for targeting therapy of large intestinal cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Peptides
/
Protein Binding
/
Binding, Competitive
/
Molecular Sequence Data
/
Base Sequence
/
Colorectal Neoplasms
/
Amino Acid Sequence
/
Peptide Library
/
Cell Line, Tumor
Type of study:
Controlled clinical trial
/
Diagnostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2008
Type:
Article
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