The Role of CD44 in the Adhesion of Human Osteosarcoma to Hyaluronic Acid / 체질인류학회지

ABSTRACT

Osteoblast expresses a sequence of extracellular matrix during differentiation, suggest that multiple adhesion mechanisms regulate osteoblast differentiation and bone development. Hyaluronic acid (HA) is a glycosaminoglycan (GAG) which mainly distribute in cartilage and extracellular matrix (ECM). HA has very high molecular weights and their structures occupy large solvent domains which give solution of high viscosity. During early development and before tissue differentiation, HA can constitute the major structural macromolecule in the ECM, where it can promote both cell proliferation and migration. CD44 is a cell surface receptor for HA. The polymorphic family of integral membrane glycoproteins CD44 is found on a wide variety of cells. CD44's function in the cell membrane is transmembrane signalling between extracellular matrix and acin filament. In present study, HOS was used as a model to examine whether HOS adhere to HA, CD44 and GAGs on cell surface participate in the adhesion to HA and there is difference in CD44 expression at that time. HOS adhered to HA in the manner of time -dependent. After incubation for 180 minutes, about 90% of cells adhered to HA. When HOS was pretreated with anti -CD44 antibody, hyaluronidase and genistein, the adhesion rate was significantly decreased. CD44 was more expressed in HOS plated on HA -coated wells than BSA -coated wells. Taken together, HOS has a adhesive affinity to HA. CD44, GAGs on cell surface and tyrosine kinases play an important role in the adhesion of HOS to HA.