Coculture of elongated neuron axon with poly (D,L-lactide-co-glycolide) biomembrane in vitro / 中华创伤杂志(英文版)

ABSTRACT

<p><b>OBJECTIVE</b>To elongate human nerve axon in culture and search for suitable support matrices for peripheral nervous system transplantation.</p><p><b>METHODS</b>Human embryo cortical neuronal cells, seeded on poly (D,L-lactide-co-glycolide) (PLGA) membrane scaffolds, were elongated with a self-made neuro-axon extending device. The growth and morphological changes of neuron axons were observed to measure axolemmal permeability after elongation. Neurofilament protein was stained by immunohistochemical technique.</p><p><b>RESULTS</b>Human embryo neuron axon could be elongated and cultured on the PLGA membrane and retain their normal form and function.</p><p><b>CONCLUSIONS</b>Three dimensional scaffolds with elongated neuron axon have the basic characteristics of artificial nerves, indicating a fundemental theory of nerve repair with elongated neuron axon.</p>