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Expression of CREB/Bcl-2 in bone marrow mononuclear cells of children with acute leukemia / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics ; (12): 177-180, 2010.
Article in Chinese | WPRIM | ID: wpr-270395
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression and role of cyclic-AMP response binding protein (CREB) and Bcl-2 in children with acute leukemia.</p><p><b>METHODS</b>Ninety-two children with acute leukemia (leukemia group) and 30 children with non-hematologic malignancies (control group) were enrolled. The mRNA and protein expression of CREB and Bcl-2 in bone marrow mononuclear cells were measured by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot.</p><p><b>RESULTS</b>The mRNA and protein expression of CREB and Bcl-2 in the leukemia group was significantly higher than that in the control group (p<0.01). There were no significant differences in the expression of CREB and Bcl-2 between acute lymphoblastic leukemia and acute myeloid leukemia subgroups. At the initial diagnosis, the mRNA and protein expression of CREB and Bcl-2 in children with extramedullary infiltration was higher than that in children without (p<0.05). In the leukemia group, the mRNA and protein expression of CREB and Bcl-2 in the complete remission subgroup was significantly lower than that in the non-complete remission subgroup (p<0.01). High mRNA expression of CREB and Bcl-2 in the leukemia group was positively correlated with peripheral blood leucocyte counts (r=0.62, 0.71 respectively, p<0.05). There was a positive correlation between mRNA and protein expression of CREB and Bcl-2 (r=0.75, 0.68 respectively; p<0.05).</p><p><b>CONCLUSIONS</b>The expression of CREB and Bcl-2 may be correlated with the pathogenesis and clinical prognosis of childhood leukemia, however, their expression may not be associated with the classification of acute leukemia.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: RNA, Messenger / Bone Marrow Cells / Leukemia / Acute Disease / Cyclic AMP Response Element-Binding Protein / Proto-Oncogene Proteins c-bcl-2 / Genetics / Metabolism Limits: Adolescent / Child / Child, preschool / Female / Humans / Infant / Male Language: Chinese Journal: Chinese Journal of Contemporary Pediatrics Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: RNA, Messenger / Bone Marrow Cells / Leukemia / Acute Disease / Cyclic AMP Response Element-Binding Protein / Proto-Oncogene Proteins c-bcl-2 / Genetics / Metabolism Limits: Adolescent / Child / Child, preschool / Female / Humans / Infant / Male Language: Chinese Journal: Chinese Journal of Contemporary Pediatrics Year: 2010 Type: Article