Effect of epidermal growth factor receptor on airway remodeling in asthmatic mice and its mechanism / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 137-140, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-270410
ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship of airway remodeling with epidermal growth factor receptor (EGFR) and heparin-binding epidermal growth factor-like growth factor (HB-EGF) levels in asthmatic mice and the effect of EGFR tyrosine kinase inhibitor (AG1478) on airway remodeling.</p><p><b>METHODS</b>Twenty-four male BALB/c mice were randomly divided into three groups normal control, asthma, AG1478-treated. Mice were sensitized and challenged with ovalbumin (OVA) and a mouse mode1 of asthma was prepared. Collagen deposition was determined in Masson-stained lung sections. Periodic acid Schiff (PAS) staining was used to observe the proliferation of goblet cells. Immunohistochemistry was used to determine the protein expression of HB-EGF. RT-PCR was used to determine the mRNA expression of HB-EGF and EGFR.</p><p><b>RESULTS</b>The characteristic changes of airway remodeling occurred in the asthma group. The expression of HB-EGF and EGFR in the epithelial cells of bronchi in the asthma group was significantly higher than that in the normal control group. Compared with the asthma group, the AG1478-treated group had decreased inflammation reactions, decreased collagen deposition and proliferation of goblet cells and lower expression of EGFR and HB-EGF.</p><p><b>CONCLUSIONS</b>EGFR tyrosine kinase inhibitor (AG1478) ameliorates the progression of airway remodeling in mice with asthma by inhibitions of EGFR and HB-EGF expression and EGFR signal pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Quinazolines
/
Asthma
/
Immunohistochemistry
/
Tyrphostins
/
Intercellular Signaling Peptides and Proteins
/
Therapeutic Uses
/
Drug Therapy
/
Airway Remodeling
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2010
Type:
Article
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