Effects of perfluorooctane sulfonate on Glu, PKC and PKA activities in mouse brain / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 466-470, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-270477
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of perfluorooctane sulfonate (PFOS) on contents of glutamate and activity of protein kinase C (PKC) and A (PKA) and ultrastructure injury in the brain of male mice and to explore the mechanism of neurotoxicity and patho-alteration resulted from PFOS.</p><p><b>METHODS</b>44 male mice were randomly divided into four groups, who were respectively orally given 0, 5, 10, 20 mg/kg PFOS for 10 days. The Glu consents in the brain of the mice was measured with spectrophotometer and protein kinases activity were measured with non-radioactive assay of protein kinase and the changes of cerebral cortex ultrastructure were observed.</p><p><b>RESULTS</b>Contents of Glu in 10 and 20 mg/kg groups were (1.57 +/- 0.11) and (1.62 +/- 0.16) mmol/g prot respectively,which was significantly increased compared with the corresponding controlled group [(1.45 +/- 0.13) mmol/g prot] (F = 39.59, P < 0.05). PKC activity in 5, 10 and 20 mg/kg BW groups were (29.05 +/- 2.89), (33.65 +/- 3.82) and (34.20 +/- 3.16) pmol x min(-1) x (mg prot)-1 respectively, which was significantly increased compared with the corresponding control group [(24.53 +/- 2.88) pmol x min(-1) x (mg prot)-1] (F = 7.75, P < 0.05). Compared with the corresponding control group, PKA in 5, 10 and 20 mg/kg BW groups increased by (24.12 +/- 3.86)%, (34.02 +/- 3.04)% and (33.42 +/- 3.71)% with a statistical significance (F = 26.27, P < 0.01). The exposed mice had cerebral cortex ultrastructure injury of cell nucleus envelope hollow.</p><p><b>CONCLUSION</b>Exposure to PFOS increases Glu contents and activity of PKC and PKA in mouse brain and induce the cerebral cortex ultrastructural injury, a possible mechanism of the neurotoxicity caused by PFOS.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Brain
/
Brain Chemistry
/
Protein Kinase C
/
Cyclic AMP-Dependent Protein Kinases
/
Alkanesulfonic Acids
/
Glutamic Acid
/
Toxicity
/
Fluorocarbons
/
Metabolism
/
Mice, Inbred Strains
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2007
Type:
Article
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