Rapid Identification of Bacterial Species Associated with Bronchiectasis via Metagenomic Approach / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 898-901, 2014.
Article
in English
| WPRIM
| ID: wpr-270525
ABSTRACT
Bronchiectasis is a chronic lung disorder and a number of bacterial pathogens are involved. However, 30%-40% of sputum and purulent samples in good quality failed to grow any pathogenic bacteria, making it difficult to confirm the pathogen. In this study, we collected bronchoalveolar lavage fluid from a bronchiectasis patient undergoing acute exacerbation, and sent for 16S rDNA pyrosequencing by a 454 GS Junior machine. Metagenomic analysis showed the composition of bacterial community in sample was complex. More than a half of reads (51.3%) were from Pseudomonas aeruginosa. This result was corresponding with the culture result but came out 2 d earlier, which is meaningful for early diagnosis and treatment. The detection with 16S rDNA pyrosequencing technology is more sensitive and rapid than routine culture, and can detect the co-infection or symbiosis in airway, giving us a novel and convenient approach to perform rapid diagnosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pseudomonas aeruginosa
/
Pseudomonas Infections
/
Time Factors
/
Bronchiectasis
/
Bronchoalveolar Lavage Fluid
/
RNA, Ribosomal, 16S
/
Chemistry
/
Early Diagnosis
/
Metagenome
/
Metagenomics
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Limits:
Female
/
Humans
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2014
Type:
Article
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