Experimental study on malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate and analysis on its methylation / 生物医学与环境科学（英文）

ABSTRACT

<p><b>OBJECTIVE</b>To establish the model of human bronchial epithelial cells (16HBE) malignant transformation induced by glycidyl methacrylate (GMA) and define the different methylation genes at different stages.</p><p><b>METHODS</b>DNA was extracted at different 16HBE malignant phases and changes of genes DNA methylation at different stages were detected using Methylation chip of 'NimbleGen HG18 CpG Promoter Microarray Methylation'. Methylation-specific PCR (MSP) was used to observe the methylation status of some genes, and then compared with the control groups.</p><p><b>RESULTS</b>The result showed that GMA induced 16HBE morphorlogical transformation at the dose of 8 µg/mL, and cell exposed to GMA had 1374 genes in protophase, 825 genes in metaphase, 1149 genes in anaphase, respectively; 30 genes are all methylation in the 3 stages; 318 genes in protophase but not in metaphase and anaphase; 272 genes in metaphase but not in protophase and anaphase; 683 genes in anaphase but not in metaphase and protophase; 73 genes in protophase and metaphase but not in anaphase; 67 genes in protophase and anaphase but not in metaphase; 59 genes in metaphase and anaphase but not in protophase.</p><p><b>CONCLUSION</b>The pattern of DNA methylation could change in the process of 16HBE induced by GMA.</p>