Molecular characterization of full-length genome of Japanese encephalitis virus genotype V isolated from Tibet, China / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 231-239, 2014.
Article
in English
| WPRIM
| ID: wpr-270609
ABSTRACT
<p><b>OBJECTIVE</b>To determine the molecular characterization of full-length genome of Japanese encephalitis virus (JEV) genotype V.</p><p><b>METHODS</b>The full-length nucleotide sequences of JEV strains isolated from different locations and sources were used in sequence and phylogenetic analysis.</p><p><b>RESULTS</b>The full-length genome of genotypes V JEV, XZ0934, and Muar strain were composed of 10 983 and 10 988 nucleotides respectively and shared a lower level of identity with JEV genotypes I-IV, ranging from 78.4% (G I, KV1899) to 79.7% (G III, JaGAr01), for the nucleotide sequences, and from 90.0% (G I, KV1899) to 91.8% (G III, JaGAr01) for the amino acid sequences. The open reading frame (ORF) of JEV genotype V spanned nucleotides 96 to 10 397 and encoded 3 433 amino acids. Interestingly, a comparison with JEV genotype I-IV revealed that 3 nucleotides (encoded with a serine residue) were inserted in the NS4A gene of JEV genotype V, and the insertion of nucleotides was also found in downstream of the ORF stop codon in 3'-untranslated region. Moreover, numerous amino acid mutations were observed in 3 functional domains of the E gene of JEV genotype V.</p><p><b>CONCLUSION</b>The molecular characterization of JEV genotype V is significantly different from that of the known genotypes I-IV. The mutations located in the coding region and the non-coding region may be molecular markers of JEV genotype V and warrant further studies to determine their effects on biology and immunogenicity of genotype V strains.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Virology
/
Molecular Sequence Data
/
Base Sequence
/
Sequence Homology, Nucleic Acid
/
Amino Acid Sequence
/
Genome, Viral
/
Sequence Analysis, DNA
/
Tibet
/
Culex
Limits:
Animals
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2014
Type:
Article
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