Three-step purification of preparative-scale antiCD20 (Fab')2 / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 622-625, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-270635
ABSTRACT
<p><b>OBJECTIVE</b>To establish a three-step purification method of preparative-scale antiCD20 (Fab')2 using AKTA prime.</p><p><b>METHODS</b>AntiCD20 (Fab')2 was extracted by hyperosmotic solution and then purified by CM sepharose FF, phenyl sepharose FF, and protein G sepharose FF.</p><p><b>RESULTS</b>Around 8 mg anti-CD20 (Fab')2, whose purification was 96.678%, was purified. The antigen-binding activity of antiCD20 (Fab')2 was similar to that of antiCD20 (Fab')2 purified by protein G sepharose FF and S-100.</p><p><b>CONCLUSION</b>The three-step purification method can obtain high-purity preparative-scale antiCD20 (Fab')2 in a simple way.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Immunoglobulin Fab Fragments
/
Chromatography
/
Antigens, CD20
/
Allergy and Immunology
/
Methods
/
Antibodies
Limits:
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2008
Type:
Article
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