Molecular characterization of vancomycin-resistant Enterococci / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 521-524, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-270658
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the homology and resistant mechanism of vancomycin-resistant Enterococci (VRE) isolates.</p><p><b>METHODS</b>A total of 9 VRE isolates were collected from 2006 to 2007 at PUMC hospital. The susceptibility of these isolates to 10 different antibiotics including vancomycin was tested by E-test. These strains were processed by brain heart infusion agar screening in the presence of vancomycin (6 microg/ml), and were analyzed for genotypic characteristics using the multiplex PCR. The homology of the isolates was determined by pulsed-field gel electrophoresis (PFGE).</p><p><b>RESULTS</b>All the 9 VRE isolates were identified as Enterococci faecium. The visual analysis of PFGE patterns revealed 6 different PFGE types. The vanA gene was confirmed by PCR and sequencing in 9 VRE isolates, which were consistent between phenotype and genotype for glycopeptides resistance.</p><p><b>CONCLUSIONS</b>Only vanA genotype was detected in PUMC hospital. Clonal dissemination, horizontal gene transfer, and the selective pressure of antimicrobial agents may contribute to the increase of VRE.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bacterial Proteins
/
Bacterial Typing Techniques
/
Classification
/
Gram-Positive Bacterial Infections
/
Enterococcus faecium
/
Vancomycin Resistance
/
Drug Resistance, Multiple, Bacterial
/
Genetics
/
Microbiology
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2008
Type:
Article
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