Expression of toll-like receptors in human epidermal keratinocytes / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 296-300, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-270703
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the toll-like receptors (TLR) profile of human epidermal keratinocytes.</p><p><b>METHODS</b>We cultured the immortalized human epidermal keratinocyte cell line HaCaT cells and normal human epidermal keratinocytes (NHEK) and separated epidermis with dispase from foreskins. TLR 1-10 mRNA expression was detected with reverse transcription polymerase chain reaction (RT-PCR). TLR 2 and 4 protein expressions on surface of HaCaT cells and NHEK were detected using flow cytometry.</p><p><b>RESULTS</b>HaCaT cells, NHEK, and epidermis all expressed TLR 1-10 mRNA with different intensity. TLR 4 protein was detected on the surfaces of HaCaT cells and NHEK, while the expression of TLR 2 protein was few.</p><p><b>CONCLUSION</b>Human epidermal keratinocytes constitutively express all TLR 1-10 mRNA, which may enable human keratinocytes to respond to a wide range of pathogenic micro-organisms.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Keratinocytes
/
Cell Line
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cell Biology
/
Cell Line, Tumor
/
Epidermis
/
Toll-Like Receptors
/
Toll-Like Receptor 2
/
Toll-Like Receptor 4
/
Flow Cytometry
Limits:
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2008
Type:
Article
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