Relationship between alpha- and beta-isoform of corticosteroid receptors and corticosteroid resistant allergic rhinitis / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
;
(12): 650-653, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-270739
ABSTRACT
<p><b>OBJECTIVE</b>To clarify the relationship between the expression of alpha- and beta-isoform of corticosteroid receptors (CS) in peripheral blood mononuclear cell (PBMC) and response to corticosteroid in patients with allergic rhinitis (AR).</p><p><b>METHODS</b>Semi-quantitative RT-PCR was used to detect the expression of CS-alpha, beta in PBMC in patients with AR and to observe the different responses to corticosteroid in controls. Immunocytochemical assay was used to detect the expression of protein of CS-alpha and CS-beta.</p><p><b>RESULTS</b>1) The expression of CS-alpha mRNA was detected in the sensitive group and the resistant group of patients with AR and the controls with CS-alpha/GAPDH mRNA (x +/- s) 1.15 +/- 0.75, 1.63 +/- 0.78, 1.27 +/- 0.51 respectively. 2) The expression of CS-beta mRNA in PBMC in the resistant group of patients with AR was significantly higher than that in the sensitive group and the controls (P < 0.05), with CS-beta/GAPDH mRNA 1.42 +/- 0.73, 0.82 +/- 0.59, 0.80 +/- 0.68 respectively. 3) The number of CS-beta-positive PBMC in the resistant group was significantly higher than that in the sensitive group and the controls (P < 0.01), with the number of CS-beta-positive PBMC 28.8% +/- 9. 9%, 5.9% +/- 3.2%, 5.5% +/- 6.8% respectively.</p><p><b>CONCLUSIONS</b>It is shown that the excessive expression of CS-beta may serve as a novel predictor of corticosteroid resistance in patients with AR.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Prognosis
/
Drug Resistance
/
RNA, Messenger
/
Leukocytes, Mononuclear
/
Receptors, Steroid
/
Case-Control Studies
/
Rhinitis, Allergic, Perennial
/
Adrenal Cortex Hormones
/
Protein Isoforms
Type of study:
Observational study
/
Prognostic study
/
Risk factors
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Year:
2007
Type:
Article
Similar
MEDLINE
...
LILACS
LIS