A differentially methylated region of the DAZ1 gene in spermatic and somatic cells / 亚洲男科学杂志(英文版)

ABSTRACT

<p><b>AIM</b>To investigate the methylation status of the deleted in azoospermia 1 (DAZ1) gene promoter region in different cell types.</p><p><b>METHODS</b>Using CpG island Searcher software, a CpG island was found in the promoter region of the DAZ1 gene. The methylation status of this region was analyzed in sperm and leukocytes by bisulfited sequencing.</p><p><b>RESULTS</b>The methylation status of the CpG island in the DAZ1 gene promoter region differed in leukocytes and sperm it was methylated in leukocytes, but unmethylated in sperm.</p><p><b>CONCLUSION</b>A differentially methylated region of the DAZ1 gene exists in spermatic and somatic cells, suggesting that methylation of this region may regulate DAZ1 gene expression in different tissues.</p>