Saposin C stimulates growth and invasion, activates p42/44 and SAPK/JNK signaling pathways of MAPK and upregulates uPA/uPAR expression in prostate cancer and stromal cells / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 147-158, 2005.
Article
in English
| WPRIM
| ID: wpr-270858
ABSTRACT
<p><b>AIM</b>To determine the effect of saposin C (a known trophic domain of prosaposin) on proliferation, migration and invasion, as well as its effect on the expression of urokinase plasmonogen activator (uPA), its receptor (uPAR) and matrix metalloproteinases (MMP)-2 and -9 in normal and malignant prostate cells. In addition, we tested whether saposin C can activate p42/44 and stress-activated protein kinase/c-Jun NH2-terminal kinase (SAPK/JNK) signal transduction pathways of the mitogen-activated protein kinase (MAPK) superfamily.</p><p><b>METHODS</b>We employed Western blot analysis, phospho-specific antibodies, cell proliferation assay, reverse transcriptase-polymerase chain reaction, in vitro kinase assays and migration and invasion to determine the effect of saposin C on various biological behaviors of prostate stromal and cancer cells.</p><p><b>RESULTS</b>Saposin C, in a cell type-specific manner, upregulates uPA/uPAR and immediate early gene c-Jun expression, stimulates cell proliferation, migration and invasion and activates p42/44 and SAPK/JNK MAPK pathways in prostate stromal and cancer cells. Normal prostate epithelial cells were not responsive to saposin C treatment in the above studies.</p><p><b>CONCLUSION</b>Saposin C functions as a multipotential modulator of diverse biological activities in prostate cancer and stromal cells. These results strongly suggest that saposin C functions as a potent growth factor for prostatic cells and may contribute to prostate carcinogenesis and/or the development of hormone-refractory prostate cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Prostatic Neoplasms
/
Urokinase-Type Plasminogen Activator
/
Signal Transduction
/
Cell Division
/
Up-Regulation
/
Stromal Cells
/
Receptors, Cell Surface
/
Reverse Transcriptase Polymerase Chain Reaction
Limits:
Humans
/
Male
Language:
English
Journal:
Asian Journal of Andrology
Year:
2005
Type:
Article
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