Identification of a novel testis-specific gene and its potential roles in testis development/spermatogenesis / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 127-137, 2005.
Article
in English
| WPRIM
| ID: wpr-270860
ABSTRACT
<p><b>AIM</b>To identify and characterize a novel gene with potential roles in testis development and spermatogenesis.</p><p><b>METHODS</b>A cDNA microarray was constructed from a human testis large insert cDNA library and hybridized with probes of human or mouse adult and fetal testes. Differentially expressed genes were isolated and sequenced. RT-PCR was used to test the tissue distribution of the genes of interest and in situ hybridization was performed to localize the gene expression in the mouse testis. A range of bioinformatical programs including Gene Runner, SMART, NCBI Blast and Emboss CpGPlot were used to characterize the new gene's feature.</p><p><b>RESULTS</b>A novel testis-specific gene, NYD-SP5, was differentially expressed in fetal and adult testes. The deduced protein structure of NYD-SP5 was found to contain an IQ motif (a short calmodulin-binding motif containing conserved Ile and Gln residues), a Carbamate kinase-like domain, a Zn-dependent exopeptidase domain and a lactate dehydrogenase (LDH) C-terminal-like domain. RT-PCR analysis revealed that NYD-SP5 was predominantly expressed in the testis but not in other 15 tissues examined. In situ hybridization and RT-PCR examinations revealed that the expression of NYD-SP5 was confined in the male germ cell but not present in the somatic cell in the testes.</p><p><b>CONCLUSION</b>NYD-SP5 is a newly found testis-specific gene with potential roles in testis development and spermatogenesis through a calmodulin-activated enzyme.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Spermatogenesis
/
Testis
/
RNA, Messenger
/
Molecular Sequence Data
/
Base Sequence
/
Proteins
/
Chemistry
/
Amino Acid Sequence
/
Sequence Homology, Amino Acid
Limits:
Animals
Language:
English
Journal:
Asian Journal of Andrology
Year:
2005
Type:
Article
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