Comparative study on density gradients and swim-up preparation techniques utilizing neat and cryopreserved spermatozoa / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 86-92, 2005.
Article
in English
| WPRIM
| ID: wpr-270867
ABSTRACT
<p><b>AIM</b>To 1) compare post-wash and post-thaw parameters of sperm processed with PureSperm density gradient technique and swim-up method; and 2) test the efficacy of two commonly available density gradient media PureSperm and ISolate.</p><p><b>METHODS</b>This prospective study used semen specimens from 22 patients. Specimens from nine patients were processed by both PureSperm density gradient and swim-up method. These specimens were then cryopreserved. Thirteen specimens were processed by both PureSperm (40 % and 80 %) and Isolate (50 % and 90 %) double density gradient techniques. The two fractions processed by both PureSperm and swim-up were analyzed for post-wash sperm characteristics. Post-thaw analysis was done after 24 hours. Sperm fractions obtained after processing with PureSperm and ISolate were compared for post-wash sperm characteristics and ROS levels.</p><p><b>RESULTS</b>Specimens prepared with PureSperm had significantly higher median total motile sperm counts (TMSC) (32.2 x 10(6) vs. 17.6 x 10(6)), recovery rates (69.2 % vs. 50.0 %), and longevity at 4 hours (83.0 % vs. 55.0 %) compared to specimen prepared by swim-up. Post-thaw specimens also had a higher recovery and longevity at 4 hours with PureSperm as compared to the swim-up. Semen specimens processed by PureSperm had significantly higher total sperm count, TMSC, and percentage recovery rates (30.0 % vs. 19.7 %) than ISolate.</p><p><b>CONCLUSION</b>Semen quality is better preserved in fresh and cryopreserved semen prepared with PureSperm density gradient compared to swim-up. A significant enrichment of sperm is observed with PureSperm compared to ISolate. Higher recovery rates of mature motile sperm obtained after PureSperm sperm preparation may be beneficial for successful ART.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sperm Motility
/
Spermatozoa
/
Cryopreservation
/
Cell Separation
/
Centrifugation, Density Gradient
/
Prospective Studies
/
Methods
Type of study:
Observational study
Limits:
Humans
/
Male
Language:
English
Journal:
Asian Journal of Andrology
Year:
2005
Type:
Article
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