Influence of nuclear factor erythroid 2-related factor 2 genotype on tumor necrosis factor-α and metalloproteinase-9 expression in spinal cord after spinal cord injury in mice / 中华外科杂志
Chinese Journal of Surgery
;
(12): 1569-1572, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-270915
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of nuclear factor erythroid 2-related factor 2 (Nrf2) as a key transcription factor of cytoprotection against inflammation in the spinal cord upregulation of matrix metalloproteinase-9 (MMP-9), tumor necrosis factor-α(TNF-α) after spinal cord injury (SCI).</p><p><b>METHODS</b>Wild-type Nrf2(+/+) and Nrf2(-/-)-deficient mice were subjected to a murine SCI model induced by the application of vascular clips (force of 10 g) to the dura after a three-level T8-T10 laminectomy. The wet/dry weight ratio was used to reflect the percentage of water content of impaired spinal cord tissue at 48 h after SCI. The mRNA levels of MMP-9 were determined using reverse-transcriptase polymerase chain reaction (RT-PCR), and the protein levels of TNF-α and MMP-9 were detected by enzyme-linked immunosorbent assay at 24 h after SCI. Furthermore, gelatin zymography analysis was used to show MMP-9 activity of spinal cord tissue at 24 h after SCI. Software SPSS 16.0 was used for the statistical analysis.</p><p><b>RESULTS</b>After SCI, spinal cord water content, the expression of TNF-α and MMP-9 all increased in both injured Nrf2(+/+) and Nrf2(-/-) mice compared with their respective sham-operated mice. However, Nrf2(-/-) mice were shown to have more severe spinal cord edema, more TNF-α expression, more production and activity of MMP-9 compared with their wild-type Nrf2(+/+) counterparts after SCI (P < 0.05).</p><p><b>CONCLUSIONS</b>The results suggest that Nrf2 plays an important protective role in limiting the spinal cord upregulation of TNF-α and MMP-9 after SCI. It may be a new therapeutic target of SCI.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spinal Cord
/
Spinal Cord Injuries
/
Tumor Necrosis Factor-alpha
/
Mice, Knockout
/
Matrix Metalloproteinase 9
/
Disease Models, Animal
/
NF-E2-Related Factor 2
/
Genetics
/
Genotype
/
Metabolism
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2010
Type:
Article
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