Biologic effect of transforming growth factor-β1 on urethra cells cultured in vitro / 中华外科杂志
Chinese Journal of Surgery
;
(12): 929-932, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-270987
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of transforming growth factor-β1 (TGF-β1) on growth controlling and the expression of connective tissue growth factor mRNA(CTGF mRNA) in urethra epithelium cells and fibroblasts cultured in vitro.</p><p><b>METHODS</b>Urethra epithelial cells and fibroblasts were cultured in vitro and identified. The fourth generation cells were divided into control group (cultured by cell medium without TGF-β1) and experimental groups(cultured by cell medium containing TGF-β1 1, 2, 4 and 8 µg/L), the vital force of cells were examined by MTT and cell counting, the expression of CTGF mRNA were examined by RT-PCR after 24 hours.</p><p><b>RESULTS</b>The optical density and cell count decreased in experimental groups of urethra epithelium cells and increased in experimental groups of fibroblasts with the concentration of TGF-β1 being heightened compared with the control group (P < 0.05). The expression of CTGF mRNA increased with the heightening concentration of TGF-β1 in all experimental groups of urethra epithelium cells and fibroblasts by RT-PCR (P < 0.05).</p><p><b>CONCLUSIONS</b>TGF-β1 can inhibit the growth of urethra epithelium cells and promote the growth of fibroblasts in vitro, it can induce the expression of CTGF mRNA in two cells above-mentioned.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Urethra
/
RNA, Messenger
/
Cell Survival
/
Cells, Cultured
/
Cell Biology
/
Epithelial Cells
/
Transforming Growth Factor beta1
/
Connective Tissue Growth Factor
/
Fibroblasts
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2010
Type:
Article
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