Inhibitory effect of tumor suppressor PTEN on cell growth of endometrial carcinoma / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 275-278, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-271002
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the inhibitory effect of tumor suppressor PTEN on cell growth of endometrial carcinoma.</p><p><b>METHODS</b>The exogenous wild PTEN cDNA via an adenoviral vector (Ad-PTEN) was introduced into Ishikawa cells. The expression of PTEN protein was detected by Western blot. The growth of Ishikawa cells was evaluated by trypan blue exclusion method and MTT.</p><p><b>RESULTS</b>The expression of PTEN protein was induced on day 1, and greatly increasing on day 3 - 5 after Ad-PTEN infection. The expression of PTEN significantly inhibited the growth of Ishikawa cells, and also significantly inhibited the growth of Ishikawa cells induced by IGF-II.</p><p><b>CONCLUSION</b>Adenovirus-mediated introduction of exogenous PTEN into human endometrial carcinoma cells can induce growth suppression. PTEN gene may be a novel therapeutic agent for endometrial carcinoma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Physiology
/
Recombination, Genetic
/
Insulin-Like Growth Factor II
/
Transfection
/
Gene Expression Regulation, Neoplastic
/
Adenoviridae
/
Endometrial Neoplasms
/
Phosphoric Monoester Hydrolases
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2004
Type:
Article
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