Analysis of p53 gene mutation in esophageal exfoliative cells / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 349-352, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-271016
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility of detecting p53 gene mutation in exfoliative esophageal cells, and compare gene mutation between precancerous lesions and normal esophageal exfoliative cells and correlate p53 gene mutation with esophageal carcinogenesis.</p><p><b>METHODS</b>Forty-eight samples (24 normal squamous epithelia and 24 severe squamous dysplasia) were obtained by balloon cytologic technique from a high incidence area, Yanting county, Sichuan Province, China in 1982. p53 gene mutations in exons 5 and 7 were analyzed by PCR-SSCP.</p><p><b>RESULTS</b>p53 genes were detected in all samples. Five samples with p53 mutation were detected in exon 7 and no mutation was detected in exon 5 in 24 severe dysplasia samples. Three of the 5 samples with mutation in exon 7 developed esophageal cancer in 1992, 1994 and 1996 respectively. No p53 gene mutation was detected in exon 5 and 7 in normal exfoliative samples.</p><p><b>CONCLUSION</b>p53 mutation may have occurred in the precancerous lesions which contributes in the initiation of human esophageal carcinogenesis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Precancerous Conditions
/
Esophageal Neoplasms
/
Carcinoma, Squamous Cell
/
Polymerase Chain Reaction
/
Exons
/
Follow-Up Studies
/
Genes, p53
/
Point Mutation
/
Polymorphism, Single-Stranded Conformational
Type of study:
Observational study
/
Prognostic study
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2004
Type:
Article
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