Development of toxin targeting to VEGF-KDR / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 78-81, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-271062
ABSTRACT
<p><b>OBJECTIVE</b>To develop toxin targeting vascular endothelial growth factor receptor II (VEGF-II/KDR) fused with a KDR-binling peptide screened from peptide library.</p><p><b>METHODS</b>By affinity to KDR molecular which expressed specifically by new born vascular endothelial cell, peptides to KDR were screened from C7 peptide library by phage display. Among them, a peptide binding to KDR with high affinity termed as P5 was selected and fused to the N-terminal of Shiga toxin subunit A (StxA). The protein (P5-StxA) was expressed in E. coli.</p><p><b>RESULTS</b>ELISA and Western blot were applied to characterize the binding interaction between the fusion protein, P5-StxA and KDR. Cytotoxicity assay showed that P5-StxA maintained similar toxicity to cell as StxA. In the model of angiogenesis, P5-StxA inhibited selectively VEGF-induced growth of preexisting vessels of the chick chorioallantoic membrane.</p><p><b>CONCLUSION</b>These studies demonstrate the small peptide, P5, maybe be used as carrier of toxin targeting to KDR.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Fusion Proteins
/
Enzyme-Linked Immunosorbent Assay
/
Blotting, Western
/
Peptide Library
/
Protein Subunits
/
Shiga Toxin
/
Vascular Endothelial Growth Factor Receptor-2
/
Metabolism
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2004
Type:
Article
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