HPLC-ESI/MS analysis of stachydrine and its metabolites in rat urine / 药学学报
Acta Pharmaceutica Sinica
;
(12): 467-470, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-271442
ABSTRACT
<p><b>AIM</b>To identify the main metabolites of stachydrine in rat.</p><p><b>METHODS</b>The ionization, cleavage and chromatographic characteristics of stachydrine were studied by using high-performance liquid chromatography-electrospray ionization ion trap tandem mass spectrometry (HPLC-ESI/MS) for the first time. These characteristics of stachydrine were used as the basis for the analyses of metabolites in rat urine. The 0 - 24 h urine samples of rats after ig 25 mg x kg(-1) stachydrine were collected and purified by using C10 solid-phase extraction cartridge, and then analyzed by HPLC-ESI/MS to identify stachydrine and its metabolites.</p><p><b>RESULTS</b>The parent drug (stachydrine), 6 phase I metabolites (N-demethyl, dehydrogenation, ring-oxidation) and 2 phase II metabolites (glycine conjugates of 2 ring-oxidation products) were identified existing in rat urine.</p><p><b>CONCLUSION</b>The presented method was proved to be sensitive, rapid, high selective and specific for the identification of stachydrine and its metabolites in rat urine.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Urine
/
Proline
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Sensitivity and Specificity
/
Rats, Wistar
/
Plant Roots
/
Spectrometry, Mass, Electrospray Ionization
/
Stachys
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2006
Type:
Article
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