Pharmacokinetic study of ketoprofen in rat by blood microdialysis technique / 药学学报
Acta Pharmaceutica Sinica
;
(12): 452-456, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-271445
ABSTRACT
<p><b>AIM</b>To investigate the in vitro recovery and influencing factors of ketoprofen in microdialysis probe, and study the pharmacokinetic of unbound ketoprofen in rat after iv administration.</p><p><b>METHODS</b>The recovery of ketoprofen was detected by a concentration difference method. After microdialysis probe was inserted into the jugular vein of male Wistar rats, the probe was infused with various concentrations perfusate. The in vivo recovery and the pharmacokinetics of unbound ketoprofen in rat were investigated. Dialysate samples were determined by HPLC.</p><p><b>RESULTS</b>The recovery detected by gain was as the same as that by loss; the recovery was independent of the drug concentration surrounding the probe. The in vitro recovery was 28.75% by concentration difference method and the in vivo recovery was (40.3 +/- 2.7) % by retrodialysis method. After i.v. administration of ketoprofen in rat, T 1/2, AUC and CL of unbound ketoprofen were (181 +/- 16) min, (112 +/- 27) microg x min x mL(-1) and (0.22 +/- 0.05) L x min(-1), respectively.</p><p><b>CONCLUSION</b>Microdialysis sampling can be used for the pharmacokinetic study of unbound ketoprofen in rat.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacokinetics
/
Anti-Inflammatory Agents, Non-Steroidal
/
Ketoprofen
/
Chromatography, High Pressure Liquid
/
Rats, Wistar
/
Microdialysis
/
Area Under Curve
/
Injections, Intravenous
/
Methods
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2006
Type:
Article
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