Screening for metronidazole-resistance associated gene fragments of Helicobacter pylori by suppression subtractive hybridization / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 465-469, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-271501
ABSTRACT
<p><b>OBJECTIVE</b>To screen metronidazole (MTZ) -resistance associated gene fragments of Helicobacter pylori (H.pylori) by suppression subtractive hybridization(SSH).</p><p><b>METHODS</b>The suppression subtractive hybridization (SSH) was used to screen the different DNA fragments between MTZ-resistant and -susceptible clinical strains of H.pylori. The resistant strains specific gene fragments were obtained by SSH and identified by dot blotting.</p><p><b>RESULT</b>Among the 120 subtractive colonies which were randomly chosen, 37 DNA fragments were different (>or=2 times) in DNA-copy numbers between resistant and susceptible strains and 17 of them were significantly different (>or=3 times). These 17 DNA fragments were sequenced subsequently. Ten of them were new sequences and the other 7 were duplicated sequences. These sequences represented respectively depeptide ABC transporter periplasmic dipeptide-binding protein (dppA), permease protein (dppB), et al.</p><p><b>CONCLUSION</b>Gene fragments specific to MTZ-resistant H. pylori strains were obtained by SSH and these genes may associated with MTZ-resistance of H.pylori.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
DNA, Bacterial
/
Chemistry
/
Helicobacter pylori
/
Cloning, Molecular
/
Sequence Analysis, DNA
/
Drug Resistance, Bacterial
/
Genetics
/
Methods
/
Metronidazole
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2007
Type:
Article
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