Dihydroartemisinin inhibits proliferation and induces apoptosis of rat glioma C6 cells / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 267-272, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-271537
ABSTRACT
<p><b>OBJECTIVE</b>To determine the effects of dihydroartemisinin (DHA) on the proliferation and apoptosis of rat glioma C6 cells.</p><p><b>METHODS</b>DHA (1~ 125 micromol/L) was added into the cultured C6 cells and incubated for 24, 48 and 72 h. The cell proliferation and viability were determined by trypan blue exclusion assay and 3-(4, 5-dimethylthiazol-2yl)-2, 5 diphenyl tetrazolium bromide (MTT) reduction assay. The apoptosis was detected by Hoechst 33342 staining. The intracellular reactive oxygen species (ROS) was measured by H(2)DCFDA oxidative reaction.</p><p><b>RESULTS</b>DHA 5 ~ 125 micromol/L inhibited the proliferation of C6 cells in concentration- and time-dependent manners, the IC50 at 48 h was 23.4 micromol/L. DHA 5 ~ 25 micromol/L induced C6 cell apoptosis (P<0.05), and 5 ~ 125 micromol/L increased the intracellular ROS (P<0.01).</p><p><b>CONCLUSION</b>DHA inhibits the proliferation and induces the apoptosis of C6 cells; its cytotoxic effect may result from the increase of intercellular ROS.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Sesquiterpenes
/
Time Factors
/
Brain Neoplasms
/
Reactive Oxygen Species
/
Apoptosis
/
Inhibitory Concentration 50
/
Artemisinins
/
Cell Line, Tumor
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2007
Type:
Article
Similar
MEDLINE
...
LILACS
LIS