Analysis on bioactivity of HIV-1 integrase by ELISA method / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 179-184, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-271553
ABSTRACT
<p><b>OBJECTIVE</b>To develop an ELISA-based method for analyzing biologic activities of HIV-1 integrase and for high throughput screening of integrase inhibitors.</p><p><b>METHODS</b>After expression, renaturation and purification of integrase, the bioactivity of integrase and the inhibition of luffin-a were evaluated with an in vitro assay based on biotin-avidin EILSA and chemiluminescent substrates.</p><p><b>RESULT</b>(1) The specific activity of the purified integrase was 54.92 units/mg of protein. (2)IC(50) (concentration causing 50% inhibition of integrase) of luffin-a was (0.63 +/- 0.026) micromol/L.</p><p><b>CONCLUSION</b>The non-radioactive assay can be used for analysis of bioactivities and high throughput screening of inhibitors of HIV-1 integrase.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Substrate Specificity
/
Enzyme-Linked Immunosorbent Assay
/
Kinetics
/
Catalysis
/
Chemistry
/
HIV Integrase
/
Dose-Response Relationship, Drug
/
Enzyme Inhibitors
/
Ribosome Inactivating Proteins, Type 1
Limits:
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2007
Type:
Article
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