Involvement of endogenous histamine in modulatory effect of morphine on seizure susceptibility in mice / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 130-154, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-271562
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the modulatory effects of morphine on the susceptibility to pentylenetetrazole-induced seizures, and the involvement of endogenous histamine in this process.</p><p><b>METHODS</b>Both the wild-type (WT) mice and histidine decarboxylase (a key enzyme for histamine biosynthesis) deficient (HDC-KO) mice were subcutaneously injected with different doses of morphine, and 1 hour later the pentylenetetrazole solution (1.5 %) was infused into the tail vein at a constant rate of 0.3 ml/min. The minimal dose of pentylenetetrazole (mg/kg) needed to induce myoclonic jerks and clonus convulsion was recorded as the thresholds of seizures.</p><p><b>RESULT</b>In WT mice, morphine dose-dependently decreased the thresholds of both myoclonic jerks and clonus convulsion. In HDC-KO mice, morphine at 10 mg/kg only significantly decreased the threshold of myoclonic jerks from (38.6 +/-2.9)mg/kg to (32.5 +/-0.7)mg/kg, but had no significant effect on the threshold of clonus convulsion [from (51.8 +/-2.1)mg/kg to (47.6 +/-1.2)mg/kg]. In addition, the value of decreased myoclonic jerks (15.8 +/-1.4)% and clonus convulsion (8.3 +/-0.9)% thresholds were much lower in HDC-KO mice than in WT mice [(26.1 +/-2.5)% and (20.8 +/-2.4)%, respectively].</p><p><b>CONCLUSION</b>Morphine can decrease the thresholds of pentylenetetrazole in induction of seizure, and the endogenous histamine may be involved in this process.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pentylenetetrazole
/
Pharmacology
/
Physiology
/
Seizures
/
Sensory Thresholds
/
Histamine
/
Random Allocation
/
Mice, Knockout
/
Disease Susceptibility
/
Dose-Response Relationship, Drug
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2007
Type:
Article
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