Specific suppression of beta-secretase gene expression by short interfering RNA in mammalian cells / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To investigate whether short interfering RNAs(siRNAs) of beta-site APP cleaving enzyme (BACE) can inhibit the expression of BACE in mammalian cells.</p><p><b>METHODS</b>The gene of EGFP, U6 promoter and beta-secretase targeting siRNA were cloned by PCR, respectively. The PCR products were inserted into plasmid pLXSN. The interfering vector pLXSN/EGFP-U6-siBACE was transferred into SK-N-SH cells to express BACE. The inhibition effect of BACE siRNA on BACE expression was investigated by fluoroscopy and immunohistochemistry method.</p><p><b>RESULT</b>The interfering vector pLXSN/EGFP-U6-siBACE was constructed successfully. The BACE siRNA inhibited the expression of BACE in the SK-N-SH cells specifically and effectively, and the production of A beta was reduced.</p><p><b>CONCLUSION</b>BACE siRNA can inhibit the expression of BACE gene of mammalian cells.</p>