Tail-suspended model simulating mouse oocytes maturation inhibited with microgravity / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 687-696, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-271708
ABSTRACT
We studied the effects of simulated microgravity on mouse oocytes maturation, and analyzed whether the tail-suspended model can be applied to investigate simulated microgravity effects on reproductive processes in female mice. Mouse oocytes were cultured in vitro with microgravity simulated by a rotating wall vessel bioreactor and by tail-suspended model, and the maturation rate of the mouse oocytes in the two models were examined in vivo. The maturation rate of mouse oocytes cultured in simulated microgravity was 8.93%, and that was 72.33% in 1g gravity. In ratio, oocyte maturation rate had no significant difference between the rotational group and control group. Microgravity simulated by the tail-suspended model inhibited mouse oocytes maturation and increased the rate of oocytes abnormity. The maturation rate of tail-suspended mouse oocytes was 14.54%, which was significantly lower than that of control group. Tail-suspended model should be an ideal model to investigate simulated microgravity effects on reproductive processes of female mice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oocytes
/
Oogenesis
/
Physiology
/
Cells, Cultured
/
Weightlessness Simulation
/
Hindlimb Suspension
/
Cell Biology
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS