Expression of reconstructed BCR-ABL-pIRES-SEA plasmids in the skeletal muscles of BALB/c mice / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 519-523, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-271741
ABSTRACT
This paper is aimed to investigate the transcription and expression of BCR-ABL-pIRES-SEA fusion gene vaccines in vivo in mice. The reconstructed plasmids (BCR-ABL-pIRES-SEA) which were developed previously in our laboratory were injected into the skeletal muscles of BALB/c mice at 14d intervals for three cycles. The transcription and expression of BCR-ABL and staphylococcal enterotoxin A (SEA) in injection site were detected using RT-PCR and immunohistological methods. The BCR-ABL/SEA mRNA and protein could be identified in the injection site of BCR-ABL-pIRES-SEA vaccinated mice. The reconstructed BCR-ABL-pIRES-SEA plasmids can effectively express gene production in the skeletal muscles of mice and have the common features of DNA vaccine.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Recombinant Fusion Proteins
/
RNA, Messenger
/
Fusion Proteins, bcr-abl
/
Muscle, Skeletal
/
Vaccines, DNA
/
Enterotoxins
/
Allergy and Immunology
/
Genetics
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2012
Type:
Article
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