Effects of multi-glycosides of tripterygium wilfordii on histological structures and c-kit expression in testes of pubertal rats / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 832-836, 2011.
Article
in Zh
| WPRIM
| ID: wpr-272457
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the short- and long-term effects of multi-glycosides of tripterygium wilfordii (GTWon) the histological structures of testes in pubertal rats and possible mechanisms.</p><p><b>METHODS</b>Forty-eight 5-week-old male Sprague-Dawley rats were randomly intragastrically administered with low-does GTW(6 g/kg daily)and high-does GTW (12 mg/kg daily) or 1% sodium carboxymethylcellulose (6 mL/kg, control group) for four weeks. The testes were sampled for detecting histological structures and c-kit expression by immunohistochemistry 24 hrs and four weeks after drug discontinuance.</p><p><b>RESULTS</b>The number of spermatogenic cells and the expression of c-kit in testes were reduced in the two GTW treatment groups 24 hrs and 4 weeks after drug discontinuance compared with those in the control group(P<0.05). Four weeks after drug discontinuance atrophy and interstitial edema of seminiferous epitheliumin in testes were observed, and the testis weight and the expression of c-kit in testes were reduced in the high-does GTW group compared with those in the control group (P<0.01). There were no significant differences in the parameters observed between the low-dose GTW and the control group 4 weeks after drug discontinuance.</p><p><b>CONCLUSIONS</b>GTW has adverse effects on testes in a dose-dependent manner in puberty rats. Low-dose GTW may cause reversible short-term injuries to testis tissues. The damage of the interstitial tissue of testes induced by high-dose GTW may be one of the causes of long-term injuries of testes.</p>
Full text:
1
Index:
WPRIM
Main subject:
Organ Size
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Pathology
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Pharmacology
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Sperm Count
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Testis
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Chemistry
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Rats, Sprague-Dawley
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Proto-Oncogene Proteins c-kit
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Tripterygium
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Dose-Response Relationship, Drug
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2011
Type:
Article