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Effects of Leukemia Inhibitory Factor Combined with Basic Fibroblast Growth Factor on Self-maintenance and Self-renewal of Human Umbilical Cord Mesenchymal Stem Cells In Vitro / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 184-190, 2016.
Article in Chinese | WPRIM | ID: wpr-272481
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of LIF combined with bFGF on the proliferation, stemness and senescence of hUC-MSC.</p><p><b>METHODS</b>Experiments were divided into 4 groups control group, in which the cells were treated with complete medium (α-MEM containing 10% FBS); group LIF, in which the cells were treated with complete medium containing 10 ng/ml LIF; group bFGF, in which the cells were treated with complete medium containing 10 ng/ml bFGF; combination group, in which the cells were treated with complete medium containing 10 ng/ml LIF and 10 ng/ml bFGF. The growth curves of hUC-MSC at passage 4 in different groups were assayed by cell counting kit 8. Cellular morphologic changes were observed under inverted phase contrast microscope; hUC-MSC senescence in different groups was detected by β-galactosidase staining. The expression of PCNA, P16, P21, P53, OCT4 and NANOG genes was detected by RT-PCR.</p><p><b>RESULTS</b>The cell growth curves of each group were similar to the S-shape; the cell proliferation rate from high to low as follows that in the combination group > group bFGF > group LIF > control group. Senescence and declining of proliferation were observed at hUC-MSC very early in control group; the cells in group LIF maintained good cellular morphology at early stage, but cell proliferation was slow and late senescence was observed; a few cells in group bFGF presented signs of senescence, but with quick proliferation; the cells in combination group grew quickly and maintained cellular morphology of hUC-MSC for long time. The LIF and bFGF up-regulated the expression of PCNA, OCT4 and NANOG, while they down-regulated the expression of P16, P21, P53, and their combinative effects were more significant.</p><p><b>CONCLUSION</b>LIF combined with bFGF not only can promote the proliferation and maintenance of stemness of hUC-MSC, but also can delay the senescence of hUC-MSC.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Organic Chemicals / Pharmacology / Umbilical Cord / Cell Cycle / Cell Differentiation / Cells, Cultured / Genes, Homeobox / Fibroblast Growth Factor 2 / Tumor Suppressor Protein p53 / Proliferating Cell Nuclear Antigen Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Organic Chemicals / Pharmacology / Umbilical Cord / Cell Cycle / Cell Differentiation / Cells, Cultured / Genes, Homeobox / Fibroblast Growth Factor 2 / Tumor Suppressor Protein p53 / Proliferating Cell Nuclear Antigen Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2016 Type: Article